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#4peaks dna full#
#4peaks dna plus#

Clean up the PCR reaction to remove excess dNTP’s and primers.

Generate PCR fragments using one dye-labeled primer and one standard primer.ĭyes that may be used are: 6FAM, NED, VIC, and PET.

Preparation of Samples for Fragment Analysis Number from the submission form written on top and the plate name written

Less than 20 samples may be submitted in tubes with the well.

The plate must be labeled with the plate name. Should be placed in column order as listed on the sequence submission sheet.

If you are submitting 20 or more samples, the template mustīe provided in a standard half-skirted 96 well plate.

Samples will not be rerun unless there is a mechanical failure of the instrument.

Samples are submitted in a half-skirted 96 well plate compatible with the 3130XL sequencer.

The Genomics Core does not provide primers for High-Throughput sequencing.

Samples are submitted as a mixture of template and primer in the amounts specified above.

Samples submitted for the High-Throughput service must meet the following requirements: Matching the sequence submission well numbers on the sequence submission sheet. Samples should be placed in column order on the plate Of a half-skirted 96-well PCR plate (Life Technologies part number N8010560 Mix 1 microliter of the template and 1 microliter of the primer at theĪppropriate concentration listed in the table above into a single well

Submission form written on top and the plate name written Less than 20 samples may be submitted in tubes with the well number from the.

The sequencing samples should be placed inĬolumn order as listed on the sequence submission sheet.

If you are submitting 20 or more samples, the template must be provided in a.

That any sequencing failures are the fault of your laboratory. Failure to submit the standard will result in the assumption

A sequencing standard reaction must be submitted with all Big Dye Terminator.

#4peaks dna plus#

Provide 2 microliters per sequencing reaction plus 5 microliters excess. Custom primer provided by customer must be 10 micromolar concentration.Primers provided by the Genomics Core Facility:.Less than 20 samples may be submitted in tubes with the well number from the submissionįorm written on top and the plate name written on the side of the tube.The templates should be placed in column order as listed If you are submitting 20 or more samples, the template must be provided in a standard.Two primers equals two sequencing reactions, etc. One template/primer set equals one sequencing reaction. Provide 10 ul of the template for each reaction desired.Samples and primer must be dissolved in distilled water.Fragment Analysis: You submit fluorescent-labeled PCR fragments for analysis.Requirements for High-Throughput sequencing service The Genomics Core adds the sequencing reagents, cycles the plate, cleans the excessĭye from the reactions and runs the plate on the sequencer. High-Throughput sequencing service: Your sample and primer are submittedĪs a mixture for each sequencing reaction in a 96-well sequencer compatible plate.Requirements for Electrophoresis only service The Genomics Core runs your reactions on the sequencer. Electrophoresis only service: Your laboratory runs the sequencing reactionsĪnd removes excess dye.Template and primer requirements for Reaction & Electrophoresis service

#4peaks dna full#

This full service option is suggested when you have less than 15 samples to sequence. Reactions of excess Big Dye Terminator dyes, and run the reactions on the sequencer. The Genomics Core will set up the sequencing reactions, clean the Reaction & Electrophoresis service: Template and primer are submitted.Failure of sequencing reactions performed outside of GCF will be assumed to be the responsibility of the performing lab except in the event of an equipment malfunction.įour options are available for submitting samples to the Genomics Core for sequencing:.

Re-enter the sample information on this web site and provide the new plate number to To request a rerun, provided enough sample and primer remain, the customer must Changing the templateĪnd/or primer constitutes a new sample submission for which you will be charged. All reruns requested by the customer of the same template and primer will be chargedįor the first and the second run if the second run is unsuccessful.Equipment failure, GCF reagent failure, or operator error causing sequenceįailure - sequence will be rerun at no additional charge.Big Dye Terminator Dye Removal at an additional cost of $2 per sample.Print request at an additional cost of $1 per sample.Expedited service at a cost of $50 per hour for sequencing outside of standard work hours.